Effects of intrathecal morphine on protein kinase C? immuno-reactivity in the spinal dorsal horn in a rat model of incisional pain
- VernacularTitle:鞘内注射吗啡对切口疼痛大鼠脊髓背角蛋白激酶C?免疫反应的影响
- Author:
Xingguo HU
;
Yunxiang ZHANG
;
Yinming ZENG
- Publication Type:Journal Article
- Keywords:
Morphine;
Injections,spinal;
Pain;
Spinal cord;
Protein kinase C
- From:
Chinese Journal of Anesthesiology
1995;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective It has been shown that protein kinase C (PKC), especially PKCy is involved in the nociceptive processing at the spinal level. This study was designed to investigate the effects of intrathecal (IT) morphine on PKCy immuno-reactivity in the spinal dorsal horn in a rat model of incisional pain. Methods Twenty-four male SD rats weighing 250-300 g were anesthetized with intraperitoneal pentobarbital 40 mg?kg-1. PE-10 catheter was inserted intrathecally to the lumbar region according to Yaksh. Five days later an incision of 1cm long was made in the plantar region of left hindpaw, parallel to the muscle under isoflurane anesthesia according to Brennan. The animals were randomly divided into 4 groups with 6 animals in each group : group Ⅰ sham-operation group received IT artificial cerebro-spinal fluid (ACSF) 20 ?l and 30 min later inhaled 1.4% isoflurane for S min but no incision was made; group Ⅱ received ACSF 20 ?l IT 30 min before incision was made; group Ⅲ post-incisional morphine group received morphine 5 ?g IT 30 min after incision and group Ⅳ pre-incisional morphine group received morphine 5 ?g IT 30 min before incision. The animals were sacrificed under general anesthesia 2 h after incision. The L4-5 segment of spinal cord was removed for determination of the expression of PKC? in the spinal dorsal horn by immuno-histochemical method.Results In group Ⅱ the PKC?-IR gray density in the spinal dorsal horn of the operated side was significantly higher than that of contralateral side and that in group Ⅰ( P
