The Factors Affecting the Collection of Allogenic Peripheral Blood Stem Cells in Donors
- VernacularTitle:影响采集异基因供者外周血干细胞的因素探讨
- Author:
Tiejun WANG
;
Jinghan LIU
;
Shuxuan MA
- Publication Type:Journal Article
- Keywords:
Allogenic-donor;
Peripheral blood stem cell;
Collection;
Blood Cell Separation
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the factors affecting the collection of allogenic peripheral blood stem cells in donors. Methods Using COBE-spectra blood cell separator to collect peripheral blood stem cells in 21 allogenic donors. The impact of the factors including the number of white blood cell(WBC) and nucleated cell(NC), and mono-nucleated cell(MNC) ratio on the collecting rate of peripheral blood stem cells was analyzed before and after collection. The output of CD 34 +stem cells stimulated by different doses of G-CSF was also analyzed. Results When the mean count of peripheral WBC and MMC in donors was (41?7.7)?10 9/L and 12?2.9% respectively, the count of collected NC was 2.7?0.8?10 8/L, and the number of the collected CD 34 + stem cells was also higher. Statistical analysis showed that the number of NC was significantly related to the collecting rate of CD 34 stem cells(P
