The Effect of Age on Proliferation of Human Mesenchymal Stem Cells in Vitro
- VernacularTitle:不同年龄段人骨髓间充质干细胞体外生长特点
- Author:
Hao ZHU
;
Qiang ZHOU
;
Jianzhong XU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Age;
Cell culture;
Proliferation
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the donor age on the growth of human mesenchymal stem cells(hMSCs) in vitro. Methods HMSCs were isolated by using density gradient centrifuge method from 39 donors of different ages (7 to 79 years old), and cultured in vitro. The adhesive cells were preserved to passage, then their growth and proliferation capability were determined. Results The hMSCs from younger donors showed a stronger capability of proliferation compared with the hMSCs from older donors. Conclusion The proliferation capability of hMSCs decreased with aging. The results have significant implications for the proposed clinical application of hMSCs.
