The Expression of Caspase-2 and Caspase-3 Proteins in Cisplatin-iuduced Apoptosis of Ovarian Caner Cell Lines A2780 and A2780/DDP
- VernacularTitle:Caspase-2与caspase-3蛋白在顺铂诱导卵巢癌细胞系A2780和A2780/DDP凋亡中的表达
- Author:
Chun FU
;
Qiuhua LIN
;
Fanggen LU
- Publication Type:Journal Article
- Keywords:
Cisplatin-resistance;
Apoptosis;
Caspase-2;
Caspase-3;
Human ovarian cancer
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of caspase-2 and caspase-3 in cisplatin-induced apoptosis in cisplatin-resistant and-sensitive human ovarian cancer cell lines, A2780/DDP and A2780. Methods A2780 and A2780/DDP cells were incubated with various doses(0,5,10,20,40?M) of cisplatin for 24,48 and 72 hours, respectively. The protein expressions of caspase-2 and caspase-3 in the ovarian cancer cell lines were detected by immunohistochemical technology. The apoptotic rate of the ovarian cancer cell lines was determined by TUNEL. Results After cisplatin treated, apoptotic rates of both 2780 and A2780/DDP cell lines increased in time-and dose-dependent manners (P
