The Effect of VEGF on the Expression of Fas and FasL in Ischemic Brain Tissues
- VernacularTitle:EGF对缺血脑组织表达Fas/Fasl的影响
- Author:
Yong LIU
;
Mingjun HU
;
Hua WANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Gene therapy;
VEGF;
Fas;
FasL
- From:
Journal of Chinese Physician
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of VEGF treating cerebral ischemia. Methods A rat model of middle cerebral artery occlusion (MCAO) was set up by nylon suture embolization. The naked plasmid DNA encoding vascular endothelial growth factor(pUCCAGGS/hVEGF 165 ) was directly injected through skull into the ischemic areas of brain. After seven days the rats were killed. RT-PCR was used to measure the expression of VEGF mRNA in the brain tissues, and immunohistochemistry was appllied to detect the expression of VEGF, Fas and Fas L in the brain tissues. Results Compared with control group, VEGF expression significantly increased in the therapeutic group(P
