The relationship between activation of TLR4 and partial hepatic ischemia/reperfusion injury in mice
- VernacularTitle:肝脏Toll样受体4的激活与小鼠肝脏部分缺血再灌注损伤的关系
- Author:
Heshui WU
;
Jinxiang ZHANG
;
Jinghui ZHANG
;
Yuan TIAN
;
Lin WANG
;
Chunyou WANG
- Publication Type:Journal Article
- Keywords:
Liver;
Reperfusion injury;
Receptor;
Endotoxin
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relation between activation of TLR4 and liver injury in partial hepatic ischemia/reperfusion (I/R) injury in mice. Methods BALB/c mice were used in a model of partial hepatic I/R injury, the distribution of TLR4 in liver was observed with immunohistochemical stains. The level of plasma ALT and endotoxin in portal vein were measured. TLR4-deficient mice (C3H/Hej) and wild type mice (C3H/Heouj) were used in a model of I/R injury, the hepatic function and serum TNF-? were observed. Results 1. After one hour ischemia the expression of TLR4 protein increased at 1 st, 3 rd h of reperfusion. especially in Kupffer cells, followed by Granno-monocyte and liver sinus epithelial cell; 2. No endotoxemia developed; 3. At 3 rd h of reperfusion, serum TNF-? was significantly higher than sham group [Hej: (152?43)pg/ml vs. (18?10)pg/ml, n=6, t=5.26, P
