Inhibitory Action of Antisense c-jun oligodeoxynucleotides on Ultraviolet B Induced Fibroblast Damage
- VernacularTitle:反义c-jun寡核苷酸对成纤维细胞中波紫外线辐射损伤的抑制作用
- Author:
Xiuzu SONG
;
Jiping XIA
;
Zhigang BI
- Publication Type:Journal Article
- Keywords:
Ultraviolet rays;
Fibroblasts;
Genes, jun;
Genes, fos;
Matrix metalloproteinases
- From:
Chinese Journal of Dermatology
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitory action of antisense c-jun oligodeoxynucleotides(ODN) on matrix metalloproteinase(MMP) expression of fibroblasts induced by ultraviolet B (UVB). Methods The c-jun and c-fos protein expression induced by UVB were measured by Western blot. The mRNA expression of c-jun was determined by reverse transcriptase polymerase chain reaction (RT-PCR) after transfection with c-jun antisense ODN. The pro-MMP-1 and MMP-3 synthesis of fibroblasts was measured by ELISA after treatment with UVB and antisense c-jun ODN. Results The UVB-induced c-jun protein expression of fibroblasts increased to 1.8, 2.6, 3.3 times compared with that of non-irradiated controls,while there was no significant change in c-fos protein expression. The pro-MMP-1 and MMP-3 synthesis induced by UVB irradiation increased obviously. After transfection with different concentrations of c-jun antisense ODN, the UVB-induced c-jun mRNA expression could be significantly inhibited(P
