Application of Biosensor Technology to Screening of Anti-endotoxin Materials in Radix Paeoniae Rubra
- VernacularTitle:生物传感器技术在筛选赤芍中抗内毒素有效成分的应用研究
- Author:
Genfa LU
;
Guangxia XIAO
- Publication Type:Journal Article
- Keywords:
biosensor technology;
Radix Paeoniae rubra;
endotoxin
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To apply the biosensor technology to screening of the best method to extract and separate anti-endotoxin effective materials in Radix Paeoniae rubra.METHODS:The affinities of samples extracted by three different methods binding to lipopolysaccride(LPS)were determined with biosensor technology.Extracted materials were incubated with0.25EU/ml endotoxin for determination of change of binding activity.RESULTS:Material extracted by water showed high binding capa?bility with LPS,after incubation with endotoxin,still had highly effective anti-endotoxin component;1∶40diluted water extract could neutralize78.1%endotoxin.Determination results by biosensor technology and traditional limulus reagent showed no dif?ference.CONCLUSION:Water extraction could obtain more anti-endotoxin effective materials.Compared with traditional methods,biosensor technology is a fast,highly effective,direct and accurate method.
