Determination of Serum Rifampin and Individualized Dose in Tuberculosis Patients with Liver Damage
- VernacularTitle:伴肝功能受损肺结核患者血清中利福平的测定及个体化给药
- Author:
Tao TAO
;
Zhenjian XING
- Publication Type:Journal Article
- Keywords:
Rifampin;
liver function damage;
tuberculosis;
individualized medication
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for determination of Rifampin in human serum,and to individualize the medication for tuberculosis patients with liver damage.METHODS:Using d-nitrophenol as the internal standard,the serum samples were extracted with methanol.HPLC condition:analytical column:Lichrospher100(4.6?m?250mm),guard column:YWG-C 18 (?4mm?40mm),the mobile phase:methanol-acetonitril-0.04mol/L KH 2 PO 4 (31.5∶31.5∶37,V/V/V),the flow rate:1ml/min,the column temperature:40℃,the wavelength of UV detection:254nm.The serum concentrations were deter?mined at given time periods after patient taking an oral dose of450mg Rifampin,and need of dosage adjustment was judged.RESULTS:The chromatography was good and not interfered by the components of the serum.The dosage of Rifampin had obvious individual difference.After adjusting the dosage,the effect increased and ADRs decreased.CONCLUSION:This is a simple,rapid,accurate,sensitive and reliable method to determine the concentration of Rifampin with a wide concentration range.It is suitable for the analysis of individualized medication of Rifampin.
