Determination of Icariin in Ruzenning Tablets by Column Chromatography-HPLC
- VernacularTitle:柱层析-高效液相色谱法测定乳增宁片中淫羊藿苷含量
- Author:
Jiejiao YANG
- Publication Type:Journal Article
- Keywords:
column chromatography-HPLC;
Ruzenning tablet;
icariin;
content determination
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for determining the content of icariin in Ruzenning tablets.METHODS:Column chromatography-HPLC was adopted.C 18 column was used with acetonitrile-water(30∶70)as mobile phase and the detection wavelength was set at270nm.RESULTS:The linear range was10?g~18?g for the icariin(Y=0.9995).The average recovery was94.6%,RSD=1.95%(n=6).CONCLUSION:The method is simple and accurate with a good reproducibility and can be used as a quantitative analysis method for this preparation.