Detection of CEA~(??)mRNA in peritoneal washings of gastric cancer and its clinical significance
- VernacularTitle:胃癌术中腹腔灌洗液的CEA~(??)mRNA的检测及意义
- Author:
Yansong ZHANG
;
Guanghua LUO
;
Jun XU
;
Jiang ZHU
;
Rongchao WANG
;
Xuan DONG
;
Xiaoying ZHANG
;
Ning XU
- Publication Type:Journal Article
- Keywords:
STOMACH NEOPLASMS/diag;
PERITONEAL WASHES;
CEAmRNA
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a more sensitive method to detect free cancer cells in peritoneal washings of gastric cancer cases during surgery. Methods The CEAmRNA levels in peritoneal washings in 65 cases of gastric cancer were detected by Real-time RT-PCR. PLC was applied simultaneously to detect free cancer cells. Negative controls included the peritoneal washings from 5 cases of benign gastric diseases and the blood samples from 5 cases of healthy adult volunteers. Results (1)CEAmRNA was not found in peritoneal washings in benign gastric diseases and in blood of healthy adult volunteers. (2)The positive percentage of free cancer cells detected by Real-time RT-PCR was 47.7%, while PLC′s was only 12.3%.(3)The positive rate of CEAmRNA showed a significant difference between gastric cancer with serosal invasion and without serosa invasion groups, between peritoneal metastasis group and no peritoneal metastasis groups, and also between stages I+II and III+IV diseases(all P
