Transplantation of bone marrow mesenchymal stem cells improves motor function in a DMD mouse model
- VernacularTitle:骨髓间质干细胞移植治疗假肥大型肌营养不良症模型鼠后运动功能的改善
- Author:
Zhong LI
;
Cheng ZHANG
;
Guojun CHEN
;
Xiaorong LIU
- Publication Type:Journal Article
- Keywords:
Muscular dystrophy, Duchenne;
dko mice;
Mesenchymal stem cells;
Transplantation;
Motor activity
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe amelioration of motor function in a Duchenne muscular dystrophy (DMD) mouse model ( dko mice) after transplantation of bone marrow mesenchymal stem cells (MSCs). METHODS: Passage fifth MSCs cultured in vitro were transplanted into dko mice by tail vein, motor functions of experimental mice and matched control mice, including traction, rotating rods, rotated wheel, upside down, turning over and walking (all were recorded by Sony digital camera) were tested 15 weeks after transplantation. The fluorescent expression of dystrophin and utrophin in gastrocnemius muscle tissue of dko mice was detected by SABC-Cy3, and average optical density of positive fibers was calculated. RESULTS: MSCs grew in colony over passage third, and there was low immunologic reaction by vein transplantation. There was dystrophin and utrophin fluorescent expression in sarcolemma of dko mice 15 weeks after transplantation, but no any fluorescent expression in controls. There was significant difference in fluorescent average optical density of positive fibers between two groups ( P
