Myoblast transplantation in mdx mice prevents muscle damage by exercise
- VernacularTitle:成肌细胞移植阻止mdx鼠运动诱导的肌损害
- Author:
Weixi ZHANG
;
Youmei XIE
;
Cheng ZHANG
;
Zhuolin LIU
;
Songlin CHEN
;
Xiaoli YAO
;
Ying ZENG
;
Xiaorong LIU
- Publication Type:Journal Article
- Keywords:
Mice;
Muscular dystrophies;
Myoblast transplantation
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe skeletal muscle damage of mdx mice after overload exercise, and protection to muscle damage induced by exercise due to myoblast transplantation (MTT). METHODS: Muscle samples of C 57 mice were minced and digested with trypsin, and myoblasts were cultured ex vivo , purified and detected by immunohistochemistry stains. The myoblasts were injected into muscle of left limb of mdx mice, whereas the right limb was injected with DMEM liquid as control. Mice were submitted to exercise for 3 days starting 1 month after MTT, and then Evans blue was injected intravenously through the tail vein. The muscle cryostat sections of mdx mice were made, and then detected the immunofluorescence of dystrophin. Under a fluorescence microscope, the number of fiber stained with Evans blue and dystrophin was counted, analyzed quantitatively with image software. RESULTS: Under a fluorescence microscope, only 10 37%?2 87% muscle fibers in the myoblast grafted muscles were stained with Evans blue. In contrast, 26 82%?14 85% muscle fibers in right control muscles were stained. Significant differences between these two groups were showed ( P
