Effects of Gensenosides Rb1 on proliferation of human acute promyelocytic leukemia (HL60) cells and expression of glucocorticoid receptor ?
- VernacularTitle:人参皂甙单体Rb1对人急性早幼粒白血病细胞系增殖及糖皮质激素受体?表达的影响
- Author:
Yong LI
;
Xiaoyan ZHU
;
Changqua LING
- Publication Type:Journal Article
- Keywords:
Saponins;
Glucocorticoids;
Leukemia, promyelocytic, acute
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of Ginsenoside Rb1(G-Rb1) on transcription of glucocorticoid receptor ? (GR?) mRNA and expression of its protein in a acute promyelocytic leukemia cell line. METHODS: Antiproliferation effects of G-Rb1 on HL60 cells were determined by MTT. The transcription of GR mRNA and expression of GR were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. RESULTS: G-Rb1 inhibited the growth of HL-60 cell line in a time-dependent and dose-dependent manner. After 36 hours treatment with 80 mg/L G-Rb1, the expression of GR? mRNA and GR protein increased. CONCLUSION: G-Rb1 exhibits the inhibitory effects on cell growth in HL60 cells, and increases the expression of GR? mRNA and protein. [
