EGCG inhibit rabbit lens epithelial cell growth by regulating PKB phosphorylation
- VernacularTitle:儿茶素通过蛋白激酶B抑制兔晶状体上皮细胞增殖
- Author:
Wenyong HUANG
;
Junwen ZENG
;
Yizhi LIU
;
Shaozhen LI
- Publication Type:Journal Article
- Keywords:
Epigallocatechin-3-gallate;
Protein Kinase B;
Lens epithelial cells
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the role of PI3K /PKB pathway in the growth inhibition effect of rabbit lens epithelial cells (LECs) induced by (-)-epigallocatechin-3-gallate (EGCG). METHODS: Rabbit LECs were obtained by tissue culture; MTT colormetric assay was used to study growth of LECs; Western blotting was used to study the kinsae phosphorylation- and nonphosphorylation- level of PKB. RESULTS: (1) When LECs were preincubated with 25, 50 ?mol/L LY294002 for 1 h, 50 ?mol/L EGCG had little influence on the proliferation of LECs. It showed significant inhibition when EGCG increased to 100, 200 ?mol/L ( P
