Shenmai injection protects rat cardiomyocytes from angiotensin Ⅱ-induced apoptosis in vitro
- VernacularTitle:参麦注射液对Ang Ⅱ诱导培养心肌细胞凋亡的影响
- Author:
Yanjing CHEN
;
Ran HAO
;
Qif HUANG
- Publication Type:Journal Article
- Keywords:
Shenmai injection;
Cardiomyocytes;
Angiotensin Ⅱ;
Apoptosis;
Calcium overload
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the protective effects of Shenmai injection, a Chinese medicine, on angiotensin Ⅱ (AngⅡ)-induced rat cardiomyocyte apoptosis in vitro and the probable mechanism. METHODS: Cultured cardiomyocytes from neonatal rats were stimulated with AngⅡ. Cell viability were measured by MTT, and apoptosis was evaluated suing Hoethst33258 fluorescent dye staining and flow cytometry. Fluo-3/AM was used to test the change in intracellular free calcium. RESULTS: It was found that incubation with AngⅡ (10 -7 mol/L) for 48 h increased cardiomyocyte apoptosis, Shenmai injection (0 5 g/L, 1 0 g/L) inceased myocyte viability ( P
