Yangxue qingnao-containing serum inhibits rat vascular smooth muscle cell proliferation induced by lysophosphatidic acid
- VernacularTitle:养血清脑血清拮抗溶血磷脂酸诱导大鼠血管平滑肌细胞增殖
- Author:
Wei CAI
;
Yi XU
- Publication Type:Journal Article
- Keywords:
Yangxueqingnao particles;
Lysophospholipids;
Muscle, smooth;
Proliferation
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of Yangxue qingnao-containing serum on rat vascular smooth muscle cell (VSMC) proliferation induced by lysophosphatidic acid (LPA). METHODS: The [ 3H]-TdR incorporation and mitogen-activated protein kinasc (MAPK) activity were measured in cultured VSMC. End product of lipid peroxidation-MDA levels were also detected. RESULTS: 1?10 -9 ,1?10 -8 and 1?10 -7 mol/L LPA enhanced the cultured VSMC [ 3H]-TdR incorporation, increased MAPK activity and MDA content in a concentration-dependent manner. 5%, 10% and 15% Yangxue qingnao-containing serum concentration-dependently inhibited the increase in VSMC [ 3H]-TdR incorporation, MAPK activity and MDA content induced by LPA. CONCLUSIONS: LPA has a stimulating effect on VSMC proliferation. The LPA-induced intracellular signal transduction may be related to MAPK activity. Yangxue qingnao can efficiently inhibit LPA -induced VSMC proliferation,MAPK activity and lipid peroxidation. [
