Activation of caspase-3 during bacterial redox protein azurin -induced apoptosis in U2OS cells
- VernacularTitle:细菌氧化还原蛋白azurin诱导U2OS细胞凋亡过程中caspase-3的活化
- Author:
Xudong MIAO
;
Disheng YANG
;
Zhaoming YE
;
Rongzhen XU
;
Guidi ZHANG
- Publication Type:Journal Article
- Keywords:
Azurin;
Caspase-3;
Apoptosis;
Osteosarcoma
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study whether caspase-3,8 is activated during azurin-induced apoptosis in U2OS cells. METHODS: AnnexinV /PI method was used to detect apoptosis. The changes of procaspase-3 were analyzed by Western blot, the changes of caspase-3 mRNA were detected by semi-quantitative RT-PCR, and caspase-3 relative activity was determined by colorimetric assay. RESULTS: After U2OS cells were treated with 0, 25, 50, 100, 200, 500 mg/L azurin for 24 h, respectively, the level of procaspase-3 protein decreased and the level of caspase-3 mRNA increased as azurin concentration increased. When the cells were treated with 100 mg/L azurin for 6, 12, 24, 48 h , respectively, the caspase-3 activity began to rise from 6 h,reached the peak at 24 h,and was still higher than the control group at 48 h ( P
