Antitumor effect of chlorophyllin in vitro
- VernacularTitle:叶绿酸铜钠抗肿瘤作用的体外实验研究
- Author:
Xiaowen DING
;
Shu ZHENG
;
Jiaping PENG
;
Qinghua L
;
Jian HUANG
- Publication Type:Journal Article
- Keywords:
Chlorophyll;
Telomerase;
Colorectal neoplasms;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC 50 value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G 1 phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibits the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G 1 phase. [
