The theory and application of fluorescence resonance energy transfer
- VernacularTitle:FRET的理论基础及应用
- Author:
Xuegang SUN
;
Lihua ZHANG
;
Yong JIANG
- Publication Type:Journal Article
- Keywords:
Fluorescence resonance energy transfer;
Protein interaction
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
The structural and functional study of protein is a major topic of current functional genomics. Fluorescence resonance energy transfer (FRET) is one of few tools available for measuring nanometer scale distances and changes in distances in vivo . FRET is an ideal technology for detection of protein conformation and protein-protein interaction by using fluorescence protein, traditional organic dyes and other dyes as probes. It uses fluorescence protein, traditional organic dyes and other dyes as its probes. The application of FRET in the determination of intracellular events would be helpful for us to understand the structure and function of biology molecules. [
