Effects of sevoflurane on voltage-gated ion channels in dorsal root ganglion cells
- VernacularTitle:七氟醚对大鼠背根神经节电压门控性离子通道的影响
- Author:
Yingwei WANG
;
Yuanchang XIONG
;
Jinjun BIAN
- Publication Type:Journal Article
- Keywords:
Ganglia, spinal;
Calcium channels;
Potassium channels, voltage-gated;
Sodium channels;
Sevoflurane
- From:
Chinese Journal of Anesthesiology
1996;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of sevoflurane on voltage-gated Na~+, K~+ and Ca~(2+) channels in rat dorsal root ganglion(DRG) cells.Methods Dorsal root ganglions were dissected from thoracic and upper lumbar segments of the spinal cord. DRG cells were prepared by digestion with collagenase and trypsin at 32-34℃ The whole cell Na~+, K~+ and Ca~(2+) currents were recorded by standard patch clamp technique. The changes in the currents from holding potential of-70 mV to test potential of 0 mV were recorded in a 10 mV increments in the presence and absence of sevoflurane. Results 0.4, 0.9 and 1.8 mmol?L~(-1) sevoflurane had no effect on Na~+ or K~+ currents but Ca~(2+) currents could be significantly suppressed by 0.4 mmol?L~(-1) sevoflurane. Conclusion Na~+ and K~+ channels in DRG cells are not involved in the spinal mechanism of sevoflurane. The inhibition of Ca~(2+) current in DRG cells by sevoflurane could be associated with the antinociceptive effect of sevoflurane at the spinal cord level.
