The Effect of Rennin-angiotensin System on Endothelin-1 and Nitric Oxide in Rat Glomerulosclerosis
- VernacularTitle:阻断肾素-血管紧张素对肾病大鼠肾小球硬化ET-1及NO的作用
- Author:
Zhequan JI
;
Cuiwen HUANG
;
Jiecheng LIANG
- Publication Type:Journal Article
- Keywords:
Glomerulosclerosis;
Endothelin-1;
Inducible nitric oxide synthase;
Renin-angiotensin system(RAS)
- From:
Journal of Chinese Physician
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation among glomerulosclerosis and endothelin-1(ET-1) and nitric oxide(NO), and the effect of blocking renin-angiotensin system(RAS) on rat glomerulosclerosis. Methods 30 SD rats underwent unilateral nephrectomy plus adriamycin (6mg/kg) injection through caudal vein to establish rat glomerulosclerosis models. These rats were divided randomly into glomerulosclerosis group (group D), Benazepril treatment group (group DB) and Losartan treatment group (group DL). Another 10 SD rats served as sham-operation group (group C). 6 weeks after treamtent, the mRNA and protein expressions of ET-1 and iNOS in renal cortex were detected using RT-PCR and Western blotting analysis respectively, and the content of fibronectin(Fn) was measured using immunohistochemical method. Results Group D occurred massive proteinuria, hypoalbuminemia and hypercholesterolemia, which had significant differences compared with group C (P
