Effect of Ingredients-added Taohongsiwutang on the Secretion of Endothelin-1 and Basic Fibroblast Growth Factor of Keratinocytes
- VernacularTitle:加味桃红四物汤对角质形成细胞分泌内皮素-1和碱性成纤维细胞生长因子的影响
- Author:
Qi WANG
;
Aie XU
;
Bing XU
;
Xiaodong WEI
;
Hong SHEN
;
Jifeng LIU
- Publication Type:Journal Article
- Keywords:
Taohongsiwutang;
Keratinocytes;
Endothelin-1;
Fibroblast growth factor 2
- From:
Chinese Journal of Dermatology
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ingredients-added Taohongsiwutang(a decoction of medicinal herbs, the decoction) on the secretion of endothelin-1 and basic fibroblast growth factor of kera-tinocytes. Methods Human keratinocytes were cultured in vitro. The viability and proliferation of kera-tinocytes treated with different concentrations of the decoction were assessed by MTT method. The concentrations of endothelin-1 and basic fibroblast growth factor in the supernatant were determined by ELISA. Results The proliferation of keratinocytes was promoted by 4.27, 5.49 and 6.1 mg/mL of the decoction, endothelin-1 secretion was stimulated by 1.83 and 5.49 mg/ml of the decoction in keratinocyte culture, but no effect on the secretion of basic fibroblast growth factor. Conclusions High concentrations of the decoction can promote the proliferation of keratinocytes. Different concentrations of the decoction can stimulate keratinocytes to secrete endothelin-1, which suggests that the decoction can stimulate keratinocytes to secrete endothelin-1 in vitro.
