Simultaneous Determination of Ferulic Acid and Tetramethylpyrazine in Rabbit Serum by RP-HPLC
- VernacularTitle:反相高效液相色谱法同时测定家兔血清中阿魏酸和川芎嗪浓度
- Author:
Jinchun SONG
- Publication Type:Journal Article
- Keywords:
ferulic acid;
tetramethylpyrazine;
serum concentration of drug;
RP-HPLC;
boiling water-bath
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of ferulic acid(FA)and TMP in serum sample.METHODS:After deprotein with boiling water-bath,RP-HPLC was performed on ODS C 18 column(250mm?4.6mm)with methanol-water(containing0.1%acetic acid)=8∶17as the mobile phase and UV detection at280nm.RESU LTS:The calibration curve of FA was linear within the range of30.2～70.8?g/ml,Y=57.51+413.12X,r=0.9974;the curve of TMP was linear within the range of10.1～50.7?g/ml,Y=41.32+308.64X,r=0.9922.CONCLUSION:Compared with the traditional extraction,the method is simple,rapid and sensitive,and can be used for simultaneous determination of the contents of active compositions in Chinese materia medica.
