Determination of Cefixime in Human Plasma with HPLC
- VernacularTitle:高效液相色谱法测定人血浆中头孢克肟浓度
- Author:
Haibo ZHANG
;
Jing ZHANG
;
Zhigao SHAO
- Publication Type:Journal Article
- Keywords:
cefixime;
HPLC;
plasma drug concentration
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for determining cefixime in human plasma.METHODS:Analytical column was Discovery C 18 (4.6mm?250mm,5?m),the mobile phase consisted of methanol-acetate buffer-triethylaming(28∶72∶0.5V/V),the flow rate was1.0ml/min.The detection was performed at UV286nm.Plasma concentrations of cefixime and internal standard(Cephradine)were determined by HPLC.RESULTS:The calibration curve was linear in the concentrations ranging from0.1to5.0?g/ml(r=0.9995).The detection limit was0.1?g/ml.The recovery was(96.63?3.17)%.The within-day RSD was less than6.10%and between-day RSD was less than6.69%.CONCLUSION:The method is simple,sensitive,accurate and suitable for determination of cefixime in human plasma and pharmacokinetic study.
