Assay of Hydrochlorothiazide Tablets by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定氢氯噻嗪片含量
- Author:
Dong ZHANG
- Publication Type:Journal Article
- Keywords:
hydrochlorothiazide tablet;
RP-HPLC;
assay of content
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for determination of the content of hydrochlorothiazide tablets.METHODS:Samples were determined by RP-HPLC on a ZORBAX SB-C 18 column with a mixture of phosphate buffer solution(pH=4.0)-methanol(80∶20)as mobile phase and UV detection at226nm.RESULTS:The calibration curves of hydrochlorothiazide was linear from0.006to0.303mg/ml(r=1.0000,n=5),the average recovery and relative standard deviation were100.1%and0.43%(n=9)respectively.CONCLUSION:The method is rapid,simple,accurate and precise.It can be used for assay of hydrochlorothiazide.
