Expression of toll-like receptor 2 in liver kupffer cells in hepatic ischemia/reperfusion injury in the mice
- VernacularTitle:小鼠肝缺血/再灌注损伤时肝脏Kupffer细胞中TLR2的表达
- Author:
Heshui WU
;
Feng WANG
;
Lin WANG
;
Jie LI
;
Jinxiang ZHANG
;
Yuan TIAN
;
Jinghui ZHANG
- Publication Type:Journal Article
- Keywords:
LIVER/blood supply;
ISCHEMIA REPERFUSION INJURY;
KUPFFER CELL;
TLR2mRNA
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the synthesis of TLR2 protein and mRNA expression in Kupffer cells(KCs). Methods BALB/C mice were used to make model of partial hepatic ischemia/reperfusion (I/R) injury. After injury, KCs were isolated with two-steps in situ perfusion technique. The synthesis of TLR2 protein was determined by flow cytometric analysis (FCM) and real time reverse transcription(polymerase) chain reaction (Real Time RT-PCR) was used to analyse its gene expression in KC. Results The expression of TLR2mRNA and synthesis of protein in KC were significantly higher in experimental group, compared with sham group (protein expression: 9.19?0.43 % vs 1.52 ?0.24%, P
