Protective effect of emodin on hepatic ischemia-reperfusion injury in the rat liver
- VernacularTitle:大黄素对肝脏缺血-再灌注损伤的保护作用
- Author:
Weihong ZHAO
;
Youcheng ZHANG
;
Xusheng LI
;
Shuqin LI
;
Zhiyong YANG
- Publication Type:Journal Article
- Keywords:
LIVER/blood supply;
ISCHEMIA REPERFUSION INJURY/prev;
EMODIN/the use
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of emodin on hepatic ischemia-reperfusion injury in the rat(liver). Methods Thirty Wistar rats were randomly devided into 3 groups:the sham-operatment group (Control group),the ischemia-reperfusion group (I/R group) and the emodin-treatment group (EMD group). By(clamping) the left and median lobes of the liver for 1 h and followed by 1 h of reperfusion,the(partial) (70%)(normothemic)(ischemia)-reperfusion injury model was established. The rats of emodin group received emodin [60mg/(kg?d)] by(oral) route for 3 days before ischemia. Blood and liver samples were obtained for(biochemical) and(histological) analysis 1 h after reperfusion. Some liver specimens were taken for flow cytometry(analysis) to assess the(mitochondrial)(membrane) potential (??m) . Rhodanmin123 and PI were used to label the cells.Results After reperfusion,(serum) levels of alanine transaminase(ALT) and liver contents of(malondialdehyde) (MDA) in the I/R group rats were increased significantly (P
