Apoptosis induced by berbamine in K562 cells correlates with the expression levels of bcr/abl gene and P210
- VernacularTitle:小檗胺诱导K562细胞凋亡及其与bcr/abl gene及P210表达的关系
- Author:
Dong WU
;
Maofang LIN
;
Xiaoying ZHAO
;
Rongzhen XU
- Publication Type:Journal Article
- Keywords:
Berbamine;
Apoptosis;
Fusion proteins, bcr-abl;
Leukemia, myeloid, chronic
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of berbamine(BER) on apoptosis in K562 cells and its possible molecular mechanisms. METHODS: The apoptosis rate was measured by flow cytometry while electron microscopy and DNA electrophoresis were used to evaluate the characteristic changes of apoptosis, RT-PCR and Western blot were used to examine the expression levels of apoptosis related gene bcr/abl and BCR/ABL protein. RESULTS: By FCM, the apoptosis rate of K562 cells treated with 8.0 mg/L BER for 24 h and 72 h increased from (29.20?3.82)% to (61.77?4.35)% (P
