Influence of hypoxia-inducible factor-1 alpha on lung cancer cell A549 growth in vitro and in vivo
- VernacularTitle:转染缺氧诱导因子-1?对肺癌细胞A549生长的影响
- Author:
Huilan ZHANG
;
Zhenxiang ZHANG
;
Yongjian XU
- Publication Type:Journal Article
- Keywords:
Hypoxia-inducible factor-1;
Proliferating cell nuclear antigen;
Lung neoplasms;
A549 cells
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of hypoxia-inducible factor-1alpha(HIF-1?) on lung cancer cells A549 growth in vitro and in vivo. METHODS: To observe the growth rate of A549 cells after HIF-1? transfected, A549 cells (1?10~6/mouse) were inoculated subcutaneously into 20 nude mice, which were randomly divided into two groups: the control group (group A, n=10), the HIF-1? transfected group (group B, n=10). The weights of subcutaneous tumor were detected. The resected specimens were made into paraffin-embedded sections. The proliferating cell nuclear antigen (PCNA) was identified by immunohistochemistry(ISH). The expressions of HIF-1?? apoptosis-related protein survivin and bcl-2 were analyzed by Western blot. RESULTS: The growth rates of the HIF-1? transfected lung cancer cells A549 were significantly increased, and more importantly, the HIF-1? transfected lung cancer cells A549 was able to enhance lung cancer growth in nude mice(P
