Differential regulating effect on estrogen receptor ? and ? expression in female rat hippocampus and cortex regions between different types of estrogen regents
- VernacularTitle:不同雌激素对大鼠海马和脑皮质区域雌激素受体?和?表达的差异性调节研究
- Author:
Min JIN
;
Hefeng HUANG
;
Fan JIN
- Publication Type:Journal Article
- Keywords:
Receptors, estrogen;
Hippocampus;
Cerebral cortex;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the regulatory effect two different estrogen reagents on expressions of estrogen receptor ? and ? in female rat hippocampus and cortex regions. METHODS: 12 cycles after ovariectomy, female rats were orally injected with premarin or progynova for 3 cycles before sacrificed. Semiquantitative RT-PCR was used to detect the ERs mRNA expression and SP immunohistochemistry was performed to measure the ERs protein distribution and expression. RESULTS: In premarin group, ER ? mRNA levels in both hippocampus and cortex tissues decreased significantly compared with control. ER ? protein level in hippocampus was lower than that in the control. However, ER ? protein level in cortex had no statistical difference. ER ? mRNA in the two regions and ER ? protein in cortex had no statistical differences compared with control, while ER ? protein level in hippocampus was higher than that in the control. In progynova group, both mRNA and protein levels of ER ? increased significantly in the two regions compared with the control, and ER ? mRNA level also increased in hippocampus, but ER ? mRNA level in cortex and ER ? protein levels in the above two regions showed no statistical differences. CONCLUSION: There were differential regulatory effects on ER ? and ER ? expression in female rat cognitive regions between the two different types of estrogen reagents, which may be one of the mechanisms of varied effects in different estrogen replacement therapy reagents.
