Effect of selective estrogen receptor modulator raloxifene on estrogen responsive element transcriptional activity in vascular endothelial cells
- VernacularTitle:雷洛昔芬对血管内皮细胞雌激素应答元件转录活性的影响
- Author:
Hong HE
;
Falin YANG
;
Yinan WU
;
Xin WANG
;
Weihua XU
- Publication Type:Journal Article
- Keywords:
Selective estrogen receptor modulator;
Raloxifene;
Vascular endothelial cells
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To assess the effect of raloxifene, a selective estrogen modulator, on the transcriptional activity of estrogen responsive element (ERE) in vascular endothelial cells. METHODS: Vascular endothelial cells were transfected with ERE-TK-Luc reporter plasmid and PRL-SV40 control plasmid via FuGENE 6. The activities of firefly luciferase and renilla luciferase were measured with dual-luciferase reporter assay system. The transcriptional activity in transfected cells treated with raloxifene was compared with that in untreated cells. Furthermore, raloxifene was used to cotreat the cells with estradiol (E_2) and the influence of raloxifene to the effect of E_2 was assessed. RESULTS: Compared to untreated cells, the luciferase activity in cells treated with raloxifene decreased and showed significance at concentration of 10~(-7)mol/L (P
