Effect of perfluorocarbon on LPS-induced activation of nuclear factor-kkkkkkB in neutrophils in vitro
- VernacularTitle:氟碳化合物对脂多糖诱导体外培养中性粒细胞NF-?B活化的影响
- Author:
Zhen SUN
;
Jing LV
;
Sujuan SHAO
- Publication Type:Journal Article
- Keywords:
Fluorocarbon;
Lipopolysaccharides;
Neutrophils;
NF-kappa B
- From:
Chinese Journal of Anesthesiology
1996;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective It has been shown that partial liquid ventilation (PLV) with perfluorocarbon (PFC) can reduce intrapulmonary aggregation of neutrophils (PMNs) and macrophages and their cytokine release during acute lung injury (ALI) . The purpose of this study was to investigate the effect of PFC on activation of nuclear factor-kappa B (NF-?B) in neutrophils induced by lipopolysacchride (LPS) .Methods PMNs were isolated from peripheral blood obtained from 36 healthy volunteers and incubated in RPM 1640 cell culture medium yielding a concentration of 1?108/ hole. The PMNs were randomly divided into two groups : (Ⅰ) LPS group in which PMNs were incubated with LPS 10?g?ml-1(n = 18) and (Ⅱ) PFC group in which 5 min after stimulation of PMNs with the same concentration of LPS (10?g?ml-1 ) , PFC was added to the cell suspension with the final concentration of PFC / cell culture medium (vol / vol) of 0.3 (n=18 ). After incubation with LPS for 3 h (T1), 8h (T2) and 24 h (T3), the cell suspensions were centrifuged. The supernatants were analyzed for TNF-? concentration and gray value of activated NF-?B was measured in the nuclear of neutrophils. Results The concentration of TNF-a and gray value of NF-?B in PFC group were significantly lower than those in LPS group at all time points ( P
