Determination of fluoroacetamide in body fluid using solid-phase extraction-gas chromatography/mass spectrometry technique
- VernacularTitle:体液中氟乙酰胺SPE-GC/MS检测
- Author:
Xilan CAI
;
Xianping LIU
;
Daming ZHANG
- Publication Type:Journal Article
- Keywords:
Forensic toxicological analysis;
Fluoroacetamide;
Acetylamine;
Solid-phase extraction;
GC/MS
- From:
Chinese Journal of Forensic Medicine
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new quantitative analytical method for determination of fluoroacetamide,a kind of raticides,in blood and urine samples using solid-phase extraction combined with GC/MS.Methods Using acetamide as the internal standard,extraction column and extraction conditions of fluoroacetamide from blood and urine samples were optimized by comparing the extraction efficiency of different solid-phase extraction columns and the effects of experimental conditions on recovery.By means of the quantitative relation between the ratio of characteristic ion peak areas of fluoroacetamide to acetamide a novel analytical method for determination of fluoroacetamide concentration was established by quantitative GC/GM technique.Results The ratio was proportional to the concentration of fluoroacetamide ranging from 5.0 to 90?g/ml with silica gel column extraction,and the detection limit was estimated to be 1.0?g/ml.The average recovery of fluoroacetamide in blood sample was 91.6%.The standard deviation was less than 7.3%.Conclusion The analytical results were in conformity with those obtained by the classical method,which could satisfy the requirement for quantitative measurement of fluoroacetamide in samples from practical cases.
