A study on the activity of hTERT promoter related tumor targeting gene on colon cancer cell line DLD-1
- VernacularTitle:端粒酶启动子肿瘤靶向基因抗大肠癌的研究
- Author:
Chao HE
;
Bin CHEN
;
Lei ZHANG
;
Wenxian HU
;
Weifeng LAO
;
Xuefeng HUANG
;
Xiaotong HU
;
Binliang FANG
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Tumor necosis factor;
TRAIL gene
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression and activity of GFP/TRAIL gene activated by the hTERT promoter on colon cancer cell line DLD-1. MethodsGFP/TRAIL gene activated by the hTERT promoter was transfected into DLD-1 with adenoviral vector,expression and apoptosis inducing ability of GFP/TRAIL protein was determined by fluorescence-activated cell sorting (FACS). [WT5”HZ]ResultsThe expression of GFP gene is 41.63% and 54.07% with either hTERT promoter or CMV promoter in DLD1 cells;GFP/TRAIL gene was able to inhibit cell growth(93.50%) and induce apoptosis(56.97%) of DLD-1 ,there was significant difference between Ad/hTERT-gTRAIL and the other two control groups (PBS and Ad/hTERT-LacZ)( P
