The anti-tumor effect of lipofectamine mediated interferon-? gene therapy on experimental colon cancer model
- VernacularTitle:脂质体介导IFN-?基因治疗荷大肠癌小鼠的实验研究
- Author:
Qiang DING
;
Wenxi WU
;
Lizong SHEN
;
Ping FAN
;
Dehua XU
;
Xinyuan LIU
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Interferon type Ⅱ;
Gene therapy;
Lipofectamine
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-tumor effect of interferon-?(IFN-?) gene therapy on colon cancer. Methods BALB/c mice were inoculated subcutaneously with CT26 (murine colon carcinoma cell line) cells to prepare an in vivo tumor model. Eight days after tumor inoculation,the tumor-bearing mice were divided into 3 groups and injected intra-tumorally with one of the following preparations: pcDNA3-IFN-?/Lipofectamine,pcDNA3 /Lipofectamine,and PBS respectively. The expression of IFN-?,the immunity function of mice,the histological changes of the tumor,the tumor volume in tumor-bearing mice were tested after gene therapy. ResultsThe level of IFN-? in the serum and the CTL activity increased significantly( P
