Simultaneous Determination of Benzoic Acid and Salicylic Acid in Compound Benzoic Acid Ointment by HPLC
- VernacularTitle:高效液相色谱法同时测定复方苯甲酸软膏中两组分含量
- Author:
Yan WANG
;
Ke MA
- Publication Type:Journal Article
- Keywords:
compound benzoic acid ointment;
benzoic acid;
salicylic acid;
HPLC
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a RP-HPLC method for simultaneous determination of benzoic acid and salicylic acid in compound benzoic acid ointment.METHODS:Chromatographic condition included Hypersil ODS column and mobile phase of a mixture of potassium dihydrogen phosphate-methanol(78∶22),the detection wavelength was254nm.RESULTS:The linear ranges of benzoic acid and salicylic acid were0.4～2.0mg/ml(r=0.9999)and0.2～1.0mg/ml(r=0.9999);the average re?coveries were98.74%(RSD=0.55%)and98.67%(RSD=0.59%);the intra-day RSDs were0.56%and0.73%(n=5);the inter-day RSDs were0.75%and0.82%(n=5)respectively.CONCLUSION:This method is simple,accurate and quick,and can be used for the quality control of the preparation.
