cDNA array in the establishment of a profile in differently expressed genes in placental tissue from cases of pregnancy induced hypertension
- VernacularTitle:基因芯片研究妊娠高血压综合征患者胎盘组织基因表达的变化
- Author:
Hong WU
;
Xilong LANG
;
Weiguo ZHONG
- Publication Type:Journal Article
- Keywords:
Pregnancy complications,cardiovascular;
Hypertension;
Placenta;
Oligonucleotide array sequence analysis
- From:
Chinese Journal of Perinatal Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a differently expressed genetic profile in placental tissues of pregnancy induced hypertension(PIH). Method The total RNAs were isolated from the tissues by Trizol and mRNA was purified by Midi Kit. The expression of 17 000 genes in placental tissues of 6 PIH and 5 normoten sive women were evaluated by cDNA array technique. Results Ninety-six differently expressed genes were identified in placental tissue of PIH compared with normal pregnancy cases. Seventy-eight genes were up-regulated and 18 were down-regulated. There were 6 unclassified genes, 8 unknown expressed sequence tag (EST) fragments and 3 cDNA fragments among these differently expressed genes. The differently expressed genes were involved in transcription and translation,cell differentiation,receptor,apoptosis,immune,metabolism and growth. Conclusion The cellular mechanism of PIH involves changes in genes expression in placental tissue. Microarray is a useful method to identify differently expressed genes in tissue.
