Expression of insulin-like growth factor-Ⅱ and its mRNA in PIH Placenta
- VernacularTitle:妊娠高血压综合征胎盘滋养细胞中胰岛素样生长因子Ⅱ及其mRNA的表达
- Author:
Zhen LI
;
Guilan LIN
;
Zhi WANG
- Publication Type:Journal Article
- Keywords:
Insulin-like growth factor Ⅱ;
Trophoblast;
Pregnancy-complications,cardiovascular;
Hypertension
- From:
Chinese Journal of Perinatal Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of insulin-like growth factor-Ⅱ( IGF-II ) and its mRNA in human placenta and to evaluate its role in the pathogenesis pregnancy induced hypertension (PIH). Methods The cellular distribution of IGF-Ⅱin normal and PIH placenta was determined by immunohistochemistry. Trophoblasts were digested,separated,and purified from placenta. IGF-ⅡmRNA expression in trophoblasts was detected by RT-PCR. The mean density was determined by image analysis technique. Results (1) IGF-Ⅱwas mostly found in syncytiotrophoblast,cytotrophoblast and amnio-chorion. The strongest immunoreactivity was observed in the syncytiotrophoblast and cytotrophoblast. (2)The mean density of PIH placenta (0.324?0.042) was significantly lower than that of normotensives (0.360?0.072,P
