Effect of amygdalin and hyperoxia on surfactant associated protein A,B,C messenger RNA of type Ⅱ alveolar epithelial cells in premature rat
- VernacularTitle:苦杏仁甙对高氧暴露早产鼠肺泡Ⅱ型细胞表面活性物质蛋白A、B、C mRNA表达的影响
- Author:
Huaping ZHU
;
Liwen CHANG
;
Wenbin LI
- Publication Type:Journal Article
- Keywords:
Hyperoxia;
Pulmonary alveoli;
Epithelial cells;
Pulmonary surfactant associated proteins;
Amygdalin;
Infant,premature
- From:
Chinese Journal of Perinatal Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effect of hyperoxia and amygdalin on surfactant associated protein messenger RNA levels of alveolar epithelial cells of premature rat lung. Methods Type Ⅱ alveolar epithelial cells (AECⅡ) were gained by primary culture from 19-days fetal rat lung. After purified,AECⅡ were randomly assigned to four groups and exposed to air or hyperoxia: air group (group Ⅰ),hyperoxia group (group Ⅱ),air plus amygdalin group (group Ⅲ),hyperoxia plus amygdalin group (group Ⅳ),Groups Ⅱ、Ⅳ were flushed the flake with 95% oxygen-5% CO 2 at 3 L/min for 10 min,then sealed and cultured for 24 hours. Groups Ⅲ,Ⅳ were added 200 ?mol/L of amygdalin at the same time. All groups were in CO 2 culture chamber (37 ℃,5% CO 2) for 24 hours,cells were harvested and extracted for total RNA by Trizol reagent. mRNA levels of SP were measured by reverse transcription polymerase chain reaction (RT-PCR). Results SP mRNA levels were significantly decreased in groupⅡ compared to groupⅠ( P
