Regulation of ATP-binding acssette transporter A1 onapolipoprotein E secretion from macrophages
- VernacularTitle:ATP结合盒转运子A1对巨噬细胞载脂蛋白E分泌的调节作用
- Author:
Xiaoqin ZHOU
;
Bei CHENG
;
Yonghui YUAN
- Publication Type:Journal Article
- Keywords:
ATP-binding cassette transporters;
Apolipoproteins E;
Macrophages
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the regulatory effect of the adenosine triphosphate binding cassette transporter A1 (ABCA1) on apolipoprotein E secretion from human THP1 macrophages.METHODS: Differentiation of THP1 macrophages from monocytes was stimulated with phorbol 12-myristate 13-acetate. The macrophages then were incubated with factors which regulate ABCA1 expression. After periods of incubation, apo E secreted in the medium and synthesized in the cell was determined with ELISA, and apo E mRNA espression was detected with Northern blot.RESULTS: An increase in apo E secretion from THP1 macrophages was observed by 8 h of incubation with 8-Br-cAMP, an activator of ABCA1 expression (P
