Expression of DNA repair gene ERCC1 and its relationship with PAH-DNA adducts in lung cancer tissues
- VernacularTitle:肺癌组织中DNA修复基因ERCC1的表达与多环芳烃 -DNA加合物的关系
- Author:
Yunnan WANG
;
Jiachun L
;
Bohang ZENG
;
Xiaonong BIN
- Publication Type:Journal Article
- Keywords:
Nucleotides;
Lung neoplasms;
DNA adducts;
Gene, ERCC1
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of nucleotide excision repair gene ERCC1 and its relationship with PAH (polycyclic aromatic hydrocarbons)-DNA adducts in lung cancer tissues. METHODS: ERCC1 mRNA expression and the PAH-induced DNA adducts were detected in 150 lung cancer tissues, 120 adjacent lung tissues without cancer cells, 40 benign lung lesions and 40 normal lung tissues. The effects of some exposure factors on the expression of ERCC1 gene and the connection between ERCC1 and PAH-DNA adduct was analyzed. RESULTS: Reduced expression levels of ERCC1 were observed in 46 of 150 (30.7%) lung cancer specimens and 1 of 40 (2.5%) normal lung tissues. Smoking may suppress the expression of ERCC1 gene. The level of PAH-DNA adduct was negatively correlated with the expression of ERCC1 gene, the Spearman coefficient was -0.648, P
