Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells
- VernacularTitle:蛋白激酶C的活性变化对人肝癌细胞增殖及端粒酶表达的影响
- Author:
Haiwei ZHANG
;
Yingru LUO
;
Peie ZHENG
;
Jianming WEN
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Proliferation;
Protein kinase C;
Telomerase
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC. [
