Effect of inhibited ubiquitin-proteasome pathway on growth and activity of telomerase in esophageal carcinoma cells
- VernacularTitle:阻断泛素-蛋白酶体通路对食管癌细胞生长及端粒酶活性的影响
- Author:
Weiguo ZHANG
;
Qingming WU
;
Jieping YU
;
Xiaohu WANG
;
Guojian XIE
;
Qiang TONG
;
Chongzhen LIU
- Publication Type:Journal Article
- Keywords:
Ubiquitin-proteasome;
Esophageal neoplasms;
Cell cycle;
Apoptosis;
Telomerase
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect on growth and activity of telomerase in esophageal carcinoma cells by inhibiting ubiquitin-proteasome pathway(UPP). METHODS: The esophageal carcinoma cell strain Eca9706 was treated with MG-132 to inhibit its UPP specially. The effect of growth suppression on cells was evaluated with MTT assay, morphologic changes of cells were observed under microscope, cell cycle and apoptosis were detected by flow cytometry (FCM). DNA fragment analysis was used to confirm the presence of apoptosis. The activity of telomerase was detected. RESULTS: MG-132 had obvious inhibitory effect on the growth of Eca9706 cells in a dose and time-dependent manner. Obvious pathologic change of cells were observed under microscope, cells became round, small and exfoliating. The FCM analysis showed that the ratio of esophageal carcinoma cells of G_1 phase increased and a obviously apoptotic sub-G_1 peak was found. Agarose electrophoresis showed marked ladder. The activity of telomerase was obviously inhibited. CONCLUSIONS: MG-132 significantly inhibits the growth and the activity of telomerase of Eca 9706 cells. These findings indicate that inhibiting UPP is a new strategy for the treatment of esophageal carcinoma. [
