Effect of YIGU capsule on IGF-I mRNA and protein expression in rat osteoblasts in vitro
- VernacularTitle:益骨胶囊含药血清对大鼠成骨细胞IGF-ImRNA及其蛋白表达的影响
- Author:
Ronghua ZHANG
;
Xiaofeng ZHU
;
Yu CAI
;
Feng HUANG
;
Keping PENG
- Publication Type:Journal Article
- Keywords:
YIGU capsule;
Osteoblasts;
Insulin-like growth factor I;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The effects of YIGU capsule on proliferation and IGF-I mRNA protein expressions in osteoblasts were studied. METHODS: (1) Forty 12-month old Sprague-Dawley female rats were divided randomly into four groups (YIGU capsule high dose group, medium dose group and low dose group; saline group), the drug-containing serum and control serum were prepared. (2) The new-born Sprague-Dawley rat osteoblasts were cultured with different YIGU capsule drug-containing serum at different concentrations and different exposure time. MTT method was used to observe proliferation of osteoblasts. (3) RT-PCR method was used to measure the relative IGF-I mRNA levels and ELISA method was used to measure IGF-I secretion at different exposure time. (4) ELISA method was used to measure IGF-I secretion at different exposure time. RESULTS: (1) Proliferation of osteoblasts was more than the control groups after 48, 72 and 96 h, respectively (P
