Protective effect of Ginkgo biloba extract on testicular tissue of diabetic rats
- VernacularTitle:银杏叶提取物对糖尿病大鼠睾丸损伤的作用
- Author:
Sunzhong MAO
;
Guorong CHEN
;
Kangfu LEI
;
Xusheng LI
;
Fang WANG
;
Jianmin LI
;
Zhouxi FANG
;
Yun HU
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus, experimental;
Testis;
Lipid peroxidation;
Nitric oxide;
Ginkgo biloba
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the protective effect of Ginkgo biloba extract (GBE) on diabetic testis and explore its possible mechanism. METHODS: Testicular structure of streptozotocin-induced diabetic rats was observed under light microscopy (LM) and transmission electron microscopy (TEM). Content of malondialdehyde (MDA), NO_2~-/NO_3~- and activity of superoxide dismutase (SOD), nitric oxide synthase (NOS) were determined in testicular homogenate. RESULTS: In diabetic rats, it was manifestated as deformation of seminiferous tubule, atrophy and shedding of germinal epithelium under LM, while expansion of smooth endoplasmic reticulum, formation of fatty vacuoles and decrease of lysosome obviously in the cytoplasm of sertoli cell under TEM, the injury of testicular tissue was improved by GBE. Compared with diabetic rats, activity of SOD increased while activity of tNOS and iNOS, content of MDA and NO_2~-/NO_3~- decreased in GBE-treated rats. CONCLUSION: GBE could effectively prevent the development of diabetic testis and the effect may be partly achieved by resisting lipid peroxidation,restraining the activity of testicular tissue iNOS and reducing the pathological alterations of NO. [
