Relationship between morphologic changes in neuron or neuroglial cells and expression of TNF-? and c-Myc in cortex after focal cerebral ischemia/reperfusion in MCAO rats
- VernacularTitle:局灶性脑缺血再灌注时神经元、胶质细胞形态变化与TNF-?、c-Myc表达相关的实验研究
- Author:
Jianpeng HU
;
Jian WANG
;
Luan GAO
;
Lei L
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Reperfusion;
Neurons;
Neuroglia;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relationship between morphologic changes in neuron or neuroglial cells and expression of tumor necrosis factor ? (TNF-?) and c-Myc in cortex after focal cerebral ischemia/reperfusion in MCAO rats. METHODS: The focal cerebral ischemia/reperfusion model was established by intraluminal thread occlusion of the middle cerebral artery (MCAO). The middle cerebral arteries of rats were occluded for 2 hours and reperfused for 1, 3 and 7 days. Using the techniques of immunohistochemical staining and optical microscopy, the morphologic changes in neuron or neuroglial cells were observed in the cortex of frontal or parietal lobe; the cell types which dynamicaly expressed TNF-?, c-Myc in the different period were also observed. RESULTS: The degeneration or necrosis of neuron or neuroglial cells were observed at the center of infarction, it was very serious at 3 d after reperfussion. Astrocyte and microglial cell proliferation were observed at the broder of infarction. TNF-? and c-Myc positive cells, most of which were astrocytes and microglial cells, increased significantly at 3 d after reperfusion. CONCLUSION: TNF-? and c-Myc may play an important role in the regulation of neuron or neuroglial cells after focal cerebral ischemia with reperfusion. [
