Fenofibrate inhibits trombin-induced endothelin-1 expression in vascular endothelial cells
- VernacularTitle:非诺贝特对血管内皮细胞凝血酶诱导的内皮素-1表达的影响
- Author:
Shiming LIU
;
Yuexia DING
;
Xiaoyun LI
;
Zhaoji LI
- Publication Type:Journal Article
- Keywords:
Fenofibrate;
Endothelin-1;
Thrombin;
Arteriosclerosis;
Endothelium
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: There has been accumulating evidence demonstrating that activators for peroxisome proliferator-activated receptor ? (PPAR?) have antiinflammatory, antiatherogenic, and vasodilatory actions. We investigated the effect of PPAR? activator, fenofibrate, on trombin-induced endothelin-1 (ET-1) expression in cultured vascular endothelial cells. METHODS: Bovine aortic endothelial cells (BAECs) were treated with the PPAR? activator, fenofibrate. The ET-1 concentrations were evaluated by radioimmunoassay. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the ET-1 mRNA expression. RESULTS: Thrombin(10U/L) induced ET-1 release in BAECs [(22.4?4.7) nmol/g protein vs control (13.2?1.6) nmol/g protein, P
