Ultimate cold preservation time in Ringer's solution for murine cardiac graft
- VernacularTitle:小鼠移植心脏保存极限的研究
- Author:
Zili YANG
;
Sheng YAN
;
Jiren YU
;
Ronghua ZHANG
;
Shusen ZHENG
- Publication Type:Journal Article
- Keywords:
Heart transplantation;
Mice;
Organ, preservation
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the ultimate preservation time of murine cardiac grafts in 4℃ Ringer's solution. METHODS: Murine cardiac grafts were implanted to the abdominal vessels heterotopically 2, 4, 6, 8 and 10 hours after cold preservation. Graft survival rate and histological morphological changes, as well as the neutrilphil, T cell, macrophage infiltration, ICAM expression were determined by immunohistochemistry. RESULTS: The cardiac function-recovery rate and 1-week graft survival rate were 100% and 83.3% in 6-hour preservation group. Compared with non-preservation control group, no more apparent histological damages, cell infiltration and ICAM expression were found. CONCLUSION: The ultimate preservation time of murine cardiac graft in 4℃ Ringer's solution was 6 hours. [
