The protective effects of pretreatment with Sini Decoction on the lungs against intestinal ischemia-reperfusion induced injury in rats
- VernacularTitle:四逆汤预先给药对大鼠肠缺血/再灌注所致急性肺损伤的作用
- Author:
Kexuan LIU
;
Chuiliang LIU
;
Weikang WU
- Publication Type:Journal Article
- Keywords:
Sini Decoction;
Intestines;
Reperfusion injury;
Respiratory distress syndrome,adult
- From:
Chinese Journal of Anesthesiology
1996;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of pretreatment with Chinese herbal Sini Decoction ( SND) on the acute lung injury induced by intestinal ischemia / reperfusion (I/R) .Methods Thirty-two healthy SD rats of both sexes weighing 275-300 g were randomly divided into four groups of 8 animals : (Ⅰ) control group in which sham operation was performed, ( Ⅱ) I/R group in which superior mesenteric artery was clamped for 1 h followed by 3 h reperfusion; (Ⅲ) and (Ⅳ) SND group 1 and 2 in which SND 3 g?kg-1 ( Ⅲ) or 6 g?kg-1 (Ⅳ) was given via gastric tube every day for 3 days before I/R. Carotid artery was cannulated for MAP monitoring. The animals were sacrificed by decapitation at the end of 3 h reperfusion. Blood was collected and the lungs were immediately removed for determination of lung water content [ (wet weight - dry weight) / wet weight ?100% ], lung NO, endothelin-1 (ET-1) and MDA contents and SOD activity, lung permeability index (BALF protein concentration/serum protein concentration) and microscopic examination. Results SND pretreatment significantly alleviated the hypotension and morphological changes of the lungs induced by intestinal I/R. Lung water content, lung permeability index and lung MDA and NO contents increased significantly whereas lung SOD activity significantly decreased in I/R group ( group Ⅱ) compared with those in control group ( P
